A fifth season consisting of thirteen episodes was originally announced to air in fall 2014. After declaring that Season 5 would be the final season, Showcase announced that it would consist of sixteen episodes separated into two parts, with the first part premiering December 7, 2014.

As part of the show's promotion, a series of motion comics, Lost Girl: The Interactive Motion Comic, was released on the official Lost Girl website. The first of these appeared on August 20, 2010. The plan was to release one chapter per month, for a total of six, during the Fall 2010 television season. Around the time of the release of No. 5, the individual chapters were made available for downloading as well as being watchable on the Lost Girl website. File formats included M4V, MP4 and WMV files. It used elements of traditional print comic books with animation and audio effects. Called a motion comic by the producers, it provided a story-telling medium which was hoped would provide information about Bo and other characters from the show, and give additional insight into the supernatural world of Lost Girl.