The second season of the American television drama series Masters of Sex premiered on July 13, 2014, and will conclude on September 28, 2014. It consists of twelve episodes and Showtime broadcasts the second season on Sundays at 10:00 pm (ET) in the United States.

The series was developed for television by Michelle Ashford and is based on the biography Masters of Sex: The Life and Times of William Masters and Virginia Johnson, the Couple Who Taught America How to Love by Thomas Maier. Masters of Sex tells the story of Dr. William Masters (Michael Sheen) and Virginia Johnson (Lizzy Caplan), two pioneering researchers of human sexuality at Washington University in St. Louis, Missouri. The second season takes place between 1958 and 1961.