Three sisters, Hozuki, Kazuki, and Hazuki, are descendants of Gailieo and have completely different personalities and tastes; they never meet eye-to-eye. One day, the girls are suddenly attacked by a mysterious organization. The organization was after the Gailieo Tesoro which Galileo Galilei was said to have discovered. Still unaware of what was going on, the sisters escape on the airship Hozuki build after the sudden attack. But it won't be so easy for these three sisters to help each other out in a pinch when they're constantly at odds with each other.

Galilei Donna (ガリレイドンナ, Garireidonna) is a television anime series produced by A-1 Pictures and directed by Yasuomi Umetsu.